3D cell-based biosensors in drug discovery programs : microtissue engineering for high throughput screening /
William Ssempa Kisaalita.
- 1st ed.
- Boca Raton : CRC Press, 2010.
- xiii, 386 p. : ill. ; 20 cm.
Includes bibliographies and index.
Biosensors and bioassays Target-driven drug discovery Comparative transcriptional profiling and proteomics Comparative structure and function Chemical microenvironmental factors Spatial and temporal microenvironmental factors Material physical property and force microenvironmental factors Proteomics as a promising tool in the search for 3D biomarkers Readout present and near future Ready-to-use commercial 3D plates Challenges to adopting 3D cultures in HTS programs Cases for 3D cultures in drug discovery Ideal case study design
"This book is based upon cutting-edge research conducted in the authors lab (Cellular Bioengineering), which over the past decade has developed a number of sophisticated techniques to facilitate use of 3D cell based assays or biosensors. This book uses data from peer-reviewed publications to conclusively justify use of 3D cell cultures in cell-based biosensors (assays) for (HTS). The majority of assays performed in accelerated drug discovery processes are biochemical in nature, but there is a growing demand for live cell-based assays. Unlike biochemical ones, cellular assays are functional approximations of in vivo biological conditions and can provide more biologically relevant information"--Provided by publisher.